Semedo, Magda C.Karmali, AminBarata, PatríciaPrata, José V.2020-06-082020-06-082011SEMEDO, Magda C.; [et al] – Adsorption of myoglobin on calixarenes and biocatalysis in organic media. Journal of Advanced Chemical Engineering. ISSN 2090-4568. Vol. 1 (2011), pp. 1-102090-4568http://hdl.handle.net/10400.21/11796Derivatives of p-tert-butylcalix[4,6,8]arene carboxylic acids were used for selective adsorption of myoglobin.Amixtureofmyoglobin,laccaseandperoxidase wasusedforextractionwithcalixarenesandonlymyoglobin was selectively extracted to organic media. Myoglobin and Mb c–calixarene exhibited pseudoactivity of peroxidase in aqueous and organic media. This protein-calixarene complex exhibited the highest specific activity of 1.37 × 10−1 U.mg protein−1 at initial pH 6.5 of myoglobin aqueous solution. Apparent kinetic parameters (V max, K m, k cat and k cat/K m) for the pseudoperoxidase activity were determined in organic media for different initial pH values of myoglobin aqueous solution by Michaelis-Menten plot. The stability of this complex was studied for different initial pH values and t1/2 values were obtained in the range of 3.5–5.2 days. The extracted Mb c in organic media was recovered into fresh aqueous solutions at alkaline pH with a recovery of pseudoperoxidase activity of over 100%.engCalixarenesProtein adsorptionMyoglobinBiocatalysis in organic mediaPseudoperoxidase activityjournal article10.4303/jace/A110401