Dias, MartaRodriguez, MargaridaVasques, C.Riesenberger, BrunaMarques, LilianaGomes, BiancaPena, PedroCervantes, RenataViegas, SusanaViegas, Carla2024-08-302024-08-302024-07Dias M, Rodriguez M, Vasques C, Riesenberger B, Marques L, Gomes B, Pena P, Cervantes R, Viegas S, Viegas C. Budget-friendly protocol for TR34/L98H and TR46/Y121F/T289A mutation detection in Aspergillus section Fumigati isolates. In: SHO 2024 [hybrid], Porto (Portugal), 4-5 de julho de 2024.http://hdl.handle.net/10400.21/17652This project was supported by FCT/MCTES UIDP/05608/2020, and UIDB/05608/2020. This work is also supported by national funds through FCT/MCTES/FSE/UE, 2023.01366.BD; UI/BD/153746/2022 and CE3C unit UIDB/00329/2020; UI/BD/151431/2021; and Instituto Politécnico de Lisboa, national support through IPL/2022/InChildhealth/BI/12M; IPL/IDI&CA2023/FoodAIIEU_ESTeSL; IPL/IDI&CA2023/ASPRisk_ESTeSL; IPL/IDI&CA2023/ARAFSawmills_ESTeSL.Aspergillus section Fumigati is one of the most common sections, in the environment. It has been found in different occupational environments, such as sawmills and waste sorting. Its cryptic species show intrinsic resistance to several antifungals. Resistance in A. fumigatus is emerging due to selective pressure caused by the prolonged use of azoles. It is often associated with mutations in the Cyp51A gene. The fungal priority pathogens list (WHO), includes A. fumigatus with critical priority. Further analysis to identify potential resistance mechanisms and mutations is needed. Objective: This evaluation aims to offer a protocol for mutation detection in Aspergillus section Fumigati isolates. It will contribute to the development of guidance that can support future occupational exposure assessments.engOccupational healthOccupational exposureAspergillus fumigatusMutation detectionProtocolFCT/MCTES/FSE/UE, 2023.01366.BDUI/BD/153746/2022CE3C unit UIDB/00329/2020UI/BD/151431/2021IPL/2022/InChildhealth/BI/12MIPL/IDI&CA2023/FoodAIIEU_ESTeSLIPL/IDI&CA2023/ASPRisk_ESTeSLIPL/IDI&CA2023/ARAFSawmills_ESTeSLFCT_UIDP/05608/2020FCT_UIDB/05608/2020conference object