Brito, CláudiaRibeiro, EdnaMarques-Ramos, AnaMendes, LuísLadeira, Carina2025-04-082025-04-082024-09Brito C, Ribeiro E, Ramos A, Mendes L, Ladeira C. A mixture design with nanoplastics and bisphenol A: cytotoxicity and genotoxicity assessment. In: 52nd Congress of the European Environmental Mutagenesis and Genomics Society (EEMGS), Rovinj (Croatia), September 23-27, 2024.http://hdl.handle.net/10400.21/21789This work was supported by the project PLASCOGEN (IPL/2021/PLASCOGEN_ESTeSL). H&TRC authors gratefully acknowledge the FCT/MCTES national support through the UIDB/05608/2020 and UIDP/05608/2020.The widespread circulation of nanoplastics (NPs) and bisphenols (BPs) in Methods the environment, inevitably results in complex interactions and associated detrimental effects, which have the potential to reach the human body by multiple routes. Considering the levels of plastic oral exposure via ingestion and the absorption of these substances in gastric tissues, NPs endorse hazardous potential given the evidence of toxic effects on a cellular level. Furthermore, the ability to act as a vector for other pollutants and its ability to be internalized by cells potentiate long-term effects. Bisphenol A (BPA) is a xenoestrogen, that exhibits hormone-like properties that mimic the effects of estrogen in the body and is widely used in consumer products. The aim of this in vitro study was to investigate the cytotoxicity and genotoxicity of polystyrene (PS)-NPs and BPA per se and in combined exposures on the gastric GP202 cell line. For that, environmentally relevant concentrations previously tested in literature were selected, for PS-NPs - 20, 100, and 200 μg/mL and BPA - 0.1, 1 ng/mL, and 0.04 ng/mL as the new reference value proposed by EFSA.engPolystyreneCytotoxicityGenotoxicityOccupational exposureFCT_UIDB/05608/2020FCT_UIDP/05608/2020IPL/2021/PLASCOGEN_ESTeSLconference poster