Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/9079
Título: Characterization of gastric cells infection by diverse Helicobacter pylori strains through Fourier-transform infrared spectroscopy
Autor: Marques, Vanda
Cunha, Bernardo
Couto, Andreia
Sampaio, Pedro
Fonseca, Luís P.
Aleixo, Sandra
Calado, Cecília R. C.
Palavras-chave: Infrared spectroscopy
Helicobacter pylori
Gastric adenocarcinoma cells
Biomarkers
Espectroscopia de infravermelho
Células de adenocarcinoma gástrico
Biomarcadores
Data: 5-Mar-2019
Editora: Elsevier
Citação: MARQUES, Vanda; [et al] – Characterization of gastric cells infection by diverse Helicobacter pylori strains through Fourier-transform infrared spectroscopy. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. ISSN: 1386-1425. Vol 210, (2019), pp. 193-202.
Resumo: The infection of Helicobacter pylori, covering 50% of the world-population, leads to diverse gastric diseases as ulcers and cancer along the life-time of the human host. To promote the discovery of biomarkers of bacterial infection, in the present work, Fourier-transform infrared spectra were acquired from adenocarcinoma gastric cells, incubated with H. pylori strains presenting different genotypes concerning the virulent factors cytotoxin associated gene A and vacuolating cytotoxin A. Defined absorbance ratios were evaluated by diverse methods of statistical inference, according to the fulfillment of the tests assumptions. It was possible to define from the gastric cells, diverse absorbance ratios enabling to discriminate: i) The infection; ii) the bacteria genotype; and iii) the gastric disease of the patients from which the bacteria were isolated. These biomarkers could fasten the knowledge of the complex infection process while promoting a platform for a new diagnostic method, rapid but also specific and sensitive towards the diagnosis of both infection and bacterial virulence.
Descrição: Este trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2016 do Instituto Politécnico de Lisboa. Código de referência IPL/2017/DrugsPlatf/ISEL
Peer review: yes
URI: http://hdl.handle.net/10400.21/9079
DOI: https://doi.org/10.1016/j.saa.2018.11.001
ISSN: 1386-1425
Versão do Editor: https://reader.elsevier.com/reader/sd/pii/S1386142518309922?token=41457B7BE2E8422F4C09F7892D3AC3EACACC59EFB1970D3C9996272875BDED5020C4BB12369D40D9592C77315A50A44A
Aparece nas colecções:ISEL - Eng. Quim. Biol. - Artigos

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