Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/7270
Título: Fostering protein-calixarene interactions: from molecular recognition to sensing
Autor: Prata, Jose V.
Barata, Patricia D.
Data: 2016
Citação: PRATA, Jose V.; BARATA, Patricia D. - Fostering protein-calixarene interactions: from molecular recognition to sensing. RSC ADVANCES. ISSN: 2046-2069. Vol. 6, N.º2, (2016), pp. 1659-1669.
Resumo: Two isomeric bis-calixarene-carbazole conjugates (CCC-1 and CCC-2) endowed with carboxylic acid functions at their lower rims have been found to display a high sensing ability (KSV up to 6 x 10(7) M-1) and selectivity toward cytochrome c, a multi-functional protein, in an aqueous-based medium. After targeting basic amino acid residues on the protein surface residing near the prosthetic heme group through electrostatic and hydrophobic interactions, a rapid photoinduced electron transfer ensues between the integrated transduction element (aryleneethynylene chromophore) of CCCs and the iron-oxidized heme of cytochrome c, enabling direct detection of the protein at nanomolar levels. Our results show that CCCs are capable of efficiently discriminating heme proteins (cytochrome c vs. myoglobin) and non-heme proteins (lysozyme) in an aqueous medium. Studies performed in two solvent systems (organic and aqueous) strongly suggest that in an organic medium a Forster-type resonance energy transfer is responsible for the observed reduction in CCCs emission upon contact with heme proteins while in an aqueous medium a specific photoinduced electron transfer mechanism prevails.
Peer review: yes
URI: http://hdl.handle.net/10400.21/7270
DOI: 10.1039/c5ra19887
ISSN: 2046-2069
Versão do Editor: http://www.rsc.org/suppdata/c5/ra/c5ra19887a/c5ra19887a1.pdf
Aparece nas colecções:ISEL - Eng. Quim. Biol. - Artigos

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