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|Título:||BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies|
|Autor:||Caetano, Liliana Aranha|
Almeida, António J.
Gonçalves, L. M. D.
|Citação:||Caetano LA, Figueiredo L, Almeida AJ, Gonçalves LMD. BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies. J Microencapsulation 2017:1-12. [Epub ahead of print]|
|Resumo:||The aim of this study was to develop a novel BCG-loaded chitosan vaccine with high association efficiency which can afford efficient interaction with APC and elicit local and Th1-type-specific immune response after intranasal administration. Chitosan-suspended BCG and BCG-loaded chitosan-alginate microparticles were prepared by ionotropic gelation. Interaction with APC was evaluated by fluorescence microscopy using rBCG-GFP. Specific immune responses were evaluated following intranasal immunisation of mice. Cellular uptake was approximately two-fold higher for chitosan-suspended BCG. A single dose of BCG-loaded microparticles or chitosan-suspended BCG by intranasal route improved Th1-type response compared with subcutaneous BCG. Chitosan-suspended BCG originated the highest mucosal response in the lungs by intranasal route. These positive results indicate that the proposed approach of whole live BCG microencapsulation in chitosan-alginate for intranasal immunisation was successful in allowing efficient interaction with APC, while improving the cellular immune response, which is of interest for local immunisation against tuberculosis.|
|Versão do Editor:||http://www.tandfonline.com/doi/abs/10.1080/02652048.2017.1316325?journalCode=imnc20|
|Aparece nas colecções:||ESTeSL - Artigos|
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|BCG-loaded chitosan microparticles_Interaction with macrophages and preliminary in vivo studies.pdf||970,29 kB||Adobe PDF||Ver/Abrir Acesso Restrito. Solicitar cópia ao autor!|
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