Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/7180
Título: BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies
Autor: Caetano, Liliana Aranha
Figueiredo, Lara
Almeida, António J.
Gonçalves, L. M. D.
Palavras-chave: BCG vaccine
Chitosan
Microencapsulation
Mucosal vaccination
Data: 2017
Citação: Caetano LA, Figueiredo L, Almeida AJ, Gonçalves LMD. BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies. J Microencapsulation 2017:1-12. [Epub ahead of print]
Resumo: The aim of this study was to develop a novel BCG-loaded chitosan vaccine with high association efficiency which can afford efficient interaction with APC and elicit local and Th1-type-specific immune response after intranasal administration. Chitosan-suspended BCG and BCG-loaded chitosan-alginate microparticles were prepared by ionotropic gelation. Interaction with APC was evaluated by fluorescence microscopy using rBCG-GFP. Specific immune responses were evaluated following intranasal immunisation of mice. Cellular uptake was approximately two-fold higher for chitosan-suspended BCG. A single dose of BCG-loaded microparticles or chitosan-suspended BCG by intranasal route improved Th1-type response compared with subcutaneous BCG. Chitosan-suspended BCG originated the highest mucosal response in the lungs by intranasal route. These positive results indicate that the proposed approach of whole live BCG microencapsulation in chitosan-alginate for intranasal immunisation was successful in allowing efficient interaction with APC, while improving the cellular immune response, which is of interest for local immunisation against tuberculosis.
Peer review: yes
URI: http://hdl.handle.net/10400.21/7180
DOI: 10.1080/02652048.2017.1316325
Versão do Editor: http://www.tandfonline.com/doi/abs/10.1080/02652048.2017.1316325?journalCode=imnc20
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