Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/5115
Título: Production, purification and characterisation of polysaccharides from pleurotus ostreatus with antitumor activity
Autor: Silva, Sara
Martins, Sónia
Karmali, Amin Mahamede Vissanji
Rosa, Eduardo
Palavras-chave: Polysaccharides
Pleurotus ostreatus
Submerged Fermentation
HPLC
FTIR
Gel Filtration Chromatography
SOD-Like Activity
In vitro Antitumour Activity
Fruiting Body
Mushrooms
Culture
Data: Jul-2012
Editora: Wiley-Blackwell
Citação: SILVA, S.; MARTINS, S.; KARMALI, A.; ROSA, E. – Production, purification and characterisation of polysaccharides from pleurotus ostreatus with antitumor activity. Journal of the Science of Food and Agriculture. ISSN: 0022-5142. Vol. 92, nr. 9 (2012), pp. 1826-1832
Resumo: Background: Mushroom polysaccharides play an important role in functional foods because they exhibit biological modulator properties such as antitumour, antiviral and antibacterial activities. The present study involved the production, purification and characterisation of intracellular and extracellular free and protein-bound polysaccharides from Pleurotus ostreatus and the investigation of their growth-inhibitory effect on human carcinoma cell lines. Results: Several fermentation parameters were obtained: batch polysaccharide productivities of 0.013 +/- 8.12 x 10-5 and 0.037 +/- 0.0005 g L-1 day-1 for intracellular and extracellular polysaccharides respectively, a maximum biomass concentration of 9.35 +/- 0.18 g L-1, Pmax = 0.935 +/- 0.018 g L-1 day-1, µmax = 0.218 +/- 0.02 day-1, YEP/X = 0.040 +/- 0.0015 g g-1 and YIP/X = 0.014 +/- 0.0003 g g-1. Some polysaccharides exhibited superoxide dismutase (SOD)-like activity of 50-200 units. Fourier transform infrared analysis of the polysaccharides revealed absorption bands characteristic of such biological macromolecules. Cytotoxicity assays showed that both intracellular and extracellular polysaccharides exhibited antitumour activity towards several tested human carcinoma cell lines in a dose-dependent manner. Conclusion - The polysaccharides of P. ostreatus exhibited high SOD-like activity, which strongly supports their biological effect on tumour cell lines. The extracellular polysaccharides presented the highest antitumour activity towards the RL95 carcinoma cell line and should be further investigated as an antitumour agent.
Peer review: yes
URI: http://hdl.handle.net/10400.21/5115
DOI: 10.1002/jsfa.5560
ISSN: 0022-5142
Aparece nas colecções:ISEL - Eng. Quim. Biol. - Artigos



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