Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/4906
Título: In vitro assessment of three dimensional dense chitosan-based structures to be as bioabsorbable implants
Autor: Oliveira, Nuno Guitian
Sirgado, Tatiana
Reis, Luís
Pinto, Luís F.
Silva, Cláudia Lobato da
Ferreira, Frederico Castelo
Rodrigues, Maria Alexandra Sousa
Palavras-chave: Chitosan
Glycerol
Bioabsorbable Implants
Enzymatic Degradation
Cytotoxicity
Mechanical Properties
Data: Dez-2014
Editora: Elsevier Science BV
Citação: OLIVEIRA, Nuno Guitian; [et al] – In vitro assessment of three dimensional dense chitosan-based structures to be as bioabsorbable implants. Journal of the Mechanical Behaviour of Biomedical Materials. ISSN: 1751-6161. Vol. 40 (2014), pp. 413-425
Resumo: Chitosan biocompatibility and biodegradability properties make this biopolymer promising for the development of advanced internal fixation devices for orthopedic applications. This work presents a detailed study on the production and characterization of three dimensional (3D) dense, non-porous, chitosan-based structures, with the ability to be processed in different shapes, and also with high strength and stiffness. Such features are crucial for the application of such 3D structures as bioabsorbable implantable devices. The influence of chitosan's molecular weight and the addition of one plasticizer (glycerol) on 3D dense chitosan-based products' biomechanical properties were explored. Several specimens were produced and in vitro studies were performed in order to assess the cytotoxicity of these specimens and their physical behavior throughout the enzymatic degradation experiments. The results point out that glycerol does not impact on cytotoxicity and has a high impact in improving mechanical properties, both elasticity and compressive strength. In addition, human mesenchymal stem/stromal cells (MSC) were used as an ex-vivo model to study cell adhesion and proliferation on these structures, showing promising results with fold increase values in total cell number similar to the ones obtained in standard cell culture flasks. (C) 2014 Elsevier Ltd. All rights reserved.
Peer review: yes
URI: http://hdl.handle.net/10400.21/4906
DOI: 10.1016/j.jmbbm.2014.09.014
ISSN: 1751-6161
1878-0180
Aparece nas colecções:ISEL - Eng. Mecan. - Artigos

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