Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/475
Título: Supercritical CO2 extraction of secondary metabolites from Agaricus blazei: experiments and modelling
Autor: Coelho, José P.
Casquinha, Lúcia
Velez, António
Karmali, Amin
Data: Mai-2009
Editora: Instituto Politécnico de Lisboa
Citação: Coelho JP, Casquinha L, Velez A, Karmali A. Supercritical CO2 extraction of secondary metabolites from Agaricus blazei: experiments and modelling. Alicerces. 2009;II(2):7-15.
Resumo: The mycelium and young fruiting bodies of Agaricus blazei were submitted to supercritical CO2 extraction, in a modified commercial flow apparatus, at temperatures from 40 to 80 ºC, pressures up to 600 bar and CO2 flow-rates from 2.0 to 9.0 g.min-1. The best extraction conditions of secondary metabolites, whereby the degree of solubilization (g extract/100 g of fungi) is the highest, was obtained with pure CO2 at 400 bar, 70 ºC and a CO2 flow rate of 5.7g.min-1. The extract in that conditions were analysed by GC-Ms. In order to increase the extraction yield of secondary metabolites, which are mostly present in glycolipid fractions, a polar compound (ethanol) was used as co-solvent in the proportions of 5 and 10 % (mol/mol). The presence of ethanol increased the yield when compared with the extraction with pure CO2. Moreover, a simple model was applied to the supercritical CO2 extraction of secondary metabolites from Agaricus blazei.
Peer review: yes
URI: http://hdl.handle.net/10400.21/475
ISSN: 1645-7943
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