Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/3114
Título: The Tetrahymena chaperonin subunit CCT eta gene is coexpressed with CCT gamma gene during cilia biogenesis and cell sexual reproduction
Autor: Cyrne, Luísa
Guerreiro, Paulo
Cardoso, Ana Cristina
Rodrigues-Pousada, Claudina
Soares, Helena
Palavras-chave: CCTη-chaperonin gene
Ciliated protozoan
Gene structure
Gene expression
Chaperonin containing TCP-1
Cloning, Molecular
DNA primers
Genes, Protozoan
Macromolecular substances
Molecular sequence data
Protozoan proteins
Recombinant proteins
Sequence homology, Amino acid
Tetrahymena pyriformis
Data: Abr-1996
Editora: Elsevier
Citação: Cyrne L, Guerreiro P, Cardoso AC, Rodrigues-Pousada C, Soares H. The Tetrahymena chaperonin subunit CCT eta gene is coexpressed with CCT gamma gene during cilia biogenesis and cell sexual reproduction. FEBS Lett. 1996;383(3):277-83.
Resumo: We report here the cloning and the characterization of the T. pyriformis CCT eta gene (TpCCT eta) and also a partial sequence of the corresponding T. thermophila gene (TtCCT eta). The TpCCt eta gene encodes a protein sharing a 60.3% identity with the mouse CCT eta. We have studied the expression of these genes in Tetrahymena exponentially growing cells, cells regenerating their cilia for different periods and during different stages of the cell sexual reproduction. These genes have similar patterns of expression to those of the previously identified TpCCt gamma gene. Indeed, the Tetrahymena CCT eta and CCT gamma genes are up-regulated at 60-120 min of cilia recovery, and in conjugation when vegetative growth was resumed and cell division took place. Our results seem to indicate that both CCT subunits play an important role in the biogenesis of the newly synthesized cilia of Tetrahymena and during its cell division.
Peer review: yes
URI: http://hdl.handle.net/10400.21/3114
ISSN: 1873-3468
Versão do Editor: http://www.sciencedirect.com/science/article/pii/0014579396002402#
Aparece nas colecções:ESTeSL - Artigos

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
The Tetrahymena chaperonin subunit CCTrl gene.pdf707,59 kBAdobe PDFVer/Abrir


FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.