Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/3089
Título: Characterization of a polyubiquitin gene in T. thermophila and of ubiquitin gene expression during sexual reproduction and under stress conditions
Autor: Guerreiro, Paulo
Rodrigues-Pousada, Claudina
Palavras-chave: Ubiquitin mRNAs
Genomic organization
Tetrahymena thermophila
Ciliate
Hyperthermic stress
Amino acid sequence
Base sequence
Cloning, Molecular
Gene dosage
Gene expression regulation
Molecular sequence data
RNA, Messenger
Sequence analysis
Data: Dez-1996
Editora: Elsevier
Citação: Guerreiro P, Rodrigues-Pousada C. Characterization of a polyubiquitin gene in T. thermophila and of ubiquitin gene expression during sexual reproduction and under stress conditions. Gene. 1996;182(1-2):183-8.
Resumo: A 5-unit polyubiquitin gene, TTU3, was isolated from a T. thermophila genomic library and sequenced. This gene presents an extra triplet coding for Phe, a AGAGA motif and a putative HSE element in its 5'-non-coding region. The ubiquitin gene expression in this ciliate was investigated by Northern blot hybridization in conjugating cells or cells under stress conditions. Exponentially growing cells express two ubiquitin mRNAs of 0.75 and 1.8 kb and a new species of 1.4 kb is induced under hyperthermic stress. During sexual reproduction of the cells (conjugation) the 1.8-kb mRNA is still transcribed whereas the steady-state population of the 0.75 mRNA transcripts is strongly diminished. Southern blot analysis suggests that ubiquitin in T. thermophila constitutes a large family of about ten members.
Peer review: yes
URI: http://hdl.handle.net/10400.21/3089
ISSN: 1879-0038
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0378111996005513
Aparece nas colecções:ESTeSL - Artigos

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
Characterization of a polyubiquitin gene.pdf429,17 kBAdobe PDFVer/Abrir


FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.