Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/3086
Título: Disruption and phenotypic analysis of six open reading frames from chromosome VII of Saccharomyces cerevisiae reveals one essential gene
Autor: Guerreiro, Paulo
Rodrigues-Pousada, Claudina
Palavras-chave: Saccharomyces cerevisiae
EUROFAN
Gene disruption
ZPR1
YAP1802
LSC2
YHR162w
Chromosomes
Gene deletion
Mutagenesis, Insertional
Open reading frames/genetics
Phenotype
Plasmids/genetics
Polymerase chain reaction
Transformation, Genetic
Data: Jun-2001
Editora: Wiley
Citação: Guerreiro P, Rodrigues-Pousada C. Disruption and phenotypic analysis of six open reading frames from chromosome VII of Saccharomyces cerevisiae reveals one essential gene. Yeast. 2001;18(9):781-7.
Resumo: Six open reading frames (ORFs) located on chromosome VII of Saccharomyces cerevisiae (YGR205w, YGR210c, YGR211w, YGR241c, YGR243w and YGR244c) were disrupted in two different genetic backgrounds using short-flanking homology (SFH) gene replacement. Sporulation and tetrad analysis showed that YGR211w, recently identified as the yeast ZPR1 gene, is an essential gene. The other five genes are non-essential, and no phenotypes could be associated to their inactivation. Two of these genes have recently been further characterized: YGR241c (YAP1802) encodes a yeast adaptor protein and YGR244c (LSC2) encodes the b-subunit of the succinyl-CoA ligase. For each ORF, a replacement cassette with long flanking regions homologous to the target locus was cloned in pUG7, and the cognate wild-type gene was cloned in pRS416.
Peer review: yes
URI: http://hdl.handle.net/10400.21/3086
ISSN: 1097-0061
Versão do Editor: http://onlinelibrary.wiley.com/doi/10.1002/yea.723/abstract
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