Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/3045
Título: Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death
Autor: Nolasco, Sofia
Bellido, Javier
Gonçalves, João
Zabala, Juan Carlos
Soares, Helena
Palavras-chave: Actin cytoskeleton
Apoptosis
Caspase 7
Cell cycle
Gene silencing
Microtubule-associated proteins
Microtubules
Molecular chaperones
Phenotype
RNA, Small interfering
Tubulin
β-Tubulin cofactor A
Molecular chaperone
Data: Jul-2005
Editora: Elsevier
Citação: Nolasco S, Bellido J, Gonçalves J, Zabala JC, Soares H. Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death. FEBS Lett. 2005;579(17):3515-24.
Resumo: Microtubules are polymers of alpha/beta-tubulin participating in essential cell functions. A multistep process involving distinct molecular chaperones and cofactors produces new tubulin heterodimers competent to polymerise. In vitro cofactor A (TBCA) interacts with beta-tubulin in a quasi-native state behaving as a molecular chaperone. We have used siRNA to silence TBCA expression in HeLa and MCF-7 mammalian cell lines. TBCA is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest. In MCF-7 cells, cell death was preceded by a change in cell shape resembling differentiation.
Peer review: yes
URI: http://hdl.handle.net/10400.21/3045
ISSN: 1873-3468
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0014579305006290
Aparece nas colecções:ESTeSL - Artigos

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