Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/3038
Título: Protection against oxidative stress through SUA7/TFIIB regulation in Saccharomyces cerevisiae
Autor: Paes de Faria, Joana
Fernandes, Lisete
Palavras-chave: TFIIB
Oxidative stress signaling
Yap1
mRNA stability
Hydrogen Peroxide/pharmacology
Mutation
Oxidants/pharmacology
Oxidative stress
Plasmids
Polymerase chain reaction
RNA stability
Saccharomyces cerevisiae
Data: Dez-2006
Editora: Elsevier
Citação: Paes de Faria J, Fernandes L. Protection against oxidative stress through SUA7/TFIIB regulation in Saccharomyces cerevisiae. Free Radic Biol Med. 2006;41(11):1684-93.
Resumo: The general transcription factor TFIIB, encoded by SUA7 in Saccharomyces cerevisiae, is required for transcription activation but apparently of a specific subset of genes, for example, linked with mitochondrial activity and hence with oxidative environments. Therefore, studying SUA7/TFIIB as a potential target of oxidative stress is fundamental. We found that controlled SUA7 expression under oxidative conditions occurs at transcriptional and mRNA stability levels. Both regulatory events are associated with the transcription activator Yap1 in distinct ways: Yap1 affects SUA7 transcription up regulation in exponentially growing cells facing oxidative signals; the absence of this activator per se contributes to increase SUA7 mRNA stability. However, unlike SUA7 mRNA, TFIIB abundance is not altered on oxidative signals. The biological impact of this preferential regulation of SUA7 mRNA pool is revealed by the partial suppression of cellular oxidative sensitivity by SUA7 overexpression, and supported by the insights on the existence of a novel RNA-binding factor, acting as an oxidative sensor, which regulates mRNA stability. Taken together the results point out a primarily cellular commitment to guarantee SUA7 mRNA levels under oxidative environments.
Peer review: yes
URI: http://hdl.handle.net/10400.21/3038
ISSN: 1873-4596
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0891584906005648
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