Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/2982
Título: Differential expression of the eukaryotic release factor 3 (eRF3/GSPT1) according to gastric cancer histological types
Autor: Malta-Vacas, Joana
Aires, C.
Costa, P.
Conde, A. R.
Ramos, S.
Martins, A. P.
Monteiro, C.
Brito, Miguel
Palavras-chave: DNA, Neoplasm/genetics
Gene dosage
Neoplasm proteins/genetics
Neoplasm proteins/metabolism
Peptide termination factors/genetics
Peptide termination factors/metabolism
RNA, Neoplasm/genetics
Reverse Transcriptase Polymerase Chain Reaction/methods
Stomach neoplasms
Up-regulation
Data: Jun-2005
Editora: BMJ
Citação: Malta-Vacas J, Aires C, Costa P, Conde AR, Ramos S, Martins AP, Monteiro C, Brito M. Differential expression of the eukaryotic release factor 3 (eRF3/GSPT1) according to gastric cancer histological types. J Clin Pathol. 2005 Jun;58(6):621-5.
Resumo: Background: There are now several lines of evidence to suggest that protein synthesis and translation factors are involved in the regulation of cell proliferation and cancer development. Aims: To investigate gene expression patterns of eukaryotic releasing factor 3 (eRF3) in gastric cancer. Methods: RNA was prepared from 25 gastric tumour biopsies and adjacent non-neoplastic mucosa. Real time TaqMan reverse transcription polymerase chain reaction (RT-PCR) was performed to measure the relative gene expression levels. DNA was isolated from tumour and normal tissues and gene dosage was determined by a quantitative real time PCR using SYBR Green dye. Results: Different histological types of gastric tumours were analysed and nine of the 25 tumours revealed eRF3/GSPT1 overexpression; moreover, eight of the 12 intestinal type carcinomas analysed overexpressed the gene, whereas eRF3/GSPT1 was overexpressed in only one of the 10 diffuse type carcinomas (Kruskal-Wallis Test; p , 0.05). No correlation was found between ploidy and transcript expression levels of eRF3/GSPT1. Overexpression of eRF3/GSPT1 was not associated with increased translation rates because the upregulation of eRF3/GSPT1 did not correlate with increased eRF1 levels. Conclusions: Overexpression of eRF3/GSPT1 in intestinal type gastric tumours may lead to an increase in the translation efficiency of specific oncogenic transcripts. Alternatively, eRF3/GSPT1 may be involved in tumorigenesis as a result of its non-translational roles, namely (dis)regulating the cell cycle, apoptosis, or transcription.
Peer review: yes
URI: http://hdl.handle.net/10400.21/2982
ISSN: 1472-4146
Versão do Editor: http://jcp.bmj.com/content/58/6/621.long
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