Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/2759
Título: Use of a-SiC:H Semiconductor-Based Transducer for Glucose Sensing through FRET Analysis
Autor: Louro, Paula
Silva, Vítor
Vieira, Manuel Augusto
Karmali, Amin Mahamede Vissanji
Vieira, Maria Manuela Almeida Carvalho
Palavras-chave: Glucose sensing
FRET
Nanosensors
Fluorescence proteins
a-SiC:H photodiodes
Multilayered heterostructures
Optical sensors
Data: 2013
Editora: Springer-Verlag Berlin
Citação: LOURO, Paula; SILVA, Vítor; VIEIRA, Manuel A.; KARMALI, A.; VIEIRA, Manuela - Use of a-SiC:H Semiconductor-Based Transducer for Glucose Sensing through FRET Analysis. Technological Innovation for the Internet of Things. ISSN 1868-4238. ISBN 978-642-37290-2. Vol. 394 (2013), p. 631-638.
Relatório da Série N.º: IFIP Advances in Information and Communication Technology
Resumo: Glucose sensing is an issue with great interest in medical and biological applications. One possible approach to glucose detection takes advantage of measuring changes in fluorescence resonance energy transfer (FRET) between a fluorescent donor and an acceptor within a protein which undergoes glucose-induced changes in conformation. This demands the detection of fluorescent signals in the visible spectrum. In this paper we analyzed the emission spectrum obtained from fluorescent labels attached to a protein which changes its conformation in the presence of glucose using a commercial spectrofluorometer. Different glucose nanosensors were used to measure the output spectra with fluorescent signals located at the cyan and yellow bands of the spectrum. A new device is presented based on multilayered a-SiC:H heterostructures to detect identical transient visible signals. The transducer consists of a p-i'(a-SiC:H)-n/p-i(a-Si:H)-n heterostructure optimized for the detection of the fluorescence resonance energy transfer between fluorophores with excitation in the violet (400 nm) and emissions in the cyan (470 nm) and yellow (588 nm) range of the spectrum. Results show that the device photocurrent signal measured under reverse bias and using appropriate steady state optical bias, allows the separate detection of the cyan and yellow fluorescence signals presented.
Peer review: yes
URI: http://hdl.handle.net/10400.21/2759
ISBN: 978-3-642-37290-2
ISSN: 1868-4238
Aparece nas colecções:ISEL - Eng. Elect. Tel. Comp. - Comunicações

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