Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/2582
Título: TBCCD1, a new centrosomal protein, is required for centrosome and Golgi apparatus positioning
Autor: Gonçalves, João
Nolasco, Sofia
Nascimento, Rute
Lopez Fanarraga, Mónica
Zabala, Juan Carlos
Soares, Helena
Palavras-chave: Cell migration
Centrosome
Golgi apparatus
Primary cilia
TBCCD1
Carrier proteins
Cell nucleus
Gene silencing
Genetic complementation test
Mitotic spindle apparatus
Molecular chaperones
RNA interference
Data: Mar-2010
Editora: European Molecular Biology Organization
Citação: Gonçalves J, Nolasco S, Nascimento R, Lopez Fanarraga M, Zabala JC, Soares H. TBCCD1, a new centrosomal protein, is required for centrosome and Golgi apparatus positioning. EMBO Rep. 2010;11(3):194-200.
Resumo: In animal cells the centrosome is positioned at the cell centre in close association with the nucleus. The mechanisms responsible for this are not completely understood. Here, we report the first characterization of human TBCC-domain containing 1 (TBCCD1), a protein related to tubulin cofactor C. TBCCD1 localizes at the centrosome and at the spindle midzone, midbody and basal bodies of primary and motile cilia. Knockdown of TBCCD1 in RPE-1 cells caused the dissociation of the centrosome from the nucleus and disorganization of the Golgi apparatus. TBCCD1-depleted cells are larger, less efficient in primary cilia assembly and their migration is slower in wound-healing assays. However, the major microtubule-nucleating activity of the centrosome is not affected by TBCCD1 silencing. We propose that TBCCD1 is a key regulator of centrosome positioning and consequently of internal cell organization.
Peer review: yes
URI: http://hdl.handle.net/10400.21/2582
ISSN: 1469-3178
Versão do Editor: http://www.nature.com/embor/journal/v11/n3/full/embor20105.html
Aparece nas colecções:ESTeSL - Artigos

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