Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/2581
Título: The influence of genetic polymorphisms in XRCC3 and ADH5 genes on the frequency of genotoxicity biomarkers in workers exposed to formaldehyde
Autor: Ladeira, Carina
Viegas, Susana
Carolino, Elisabete
Gomes, Manuel C.
Brito, Miguel
Palavras-chave: Genetic susceptibility
Genotoxicity biomarkers
Occupational exposure
Aldehyde oxidoreductases/genetics
DNA repair/genetics
Environmental monitoring
Formaldehyde
Toxicity
Micronuclei, chromosome-defective
Workplace
Data: Abr-2013
Editora: Wiley-Blackwell
Citação: Ladeira C, Viegas S, Carolino E, Gomes MC, Brito M. The influence of genetic polymorphisms in XRCC3 and ADH5 genes on the frequency of genotoxicity biomarkers in workers exposed to formaldehyde. Environ Mol Mutagen. 2013;54(3):213-21.
Resumo: The International Agency for Research on Cancer classified formaldehyde as carcinogenic to humans because there is “sufficient epidemiological evidence that it causes nasopharyngeal cancer in humans”. Genes involved in DNA repair and maintenance of genome integrity are critically involved in protecting against mutations that lead to cancer and/or inherited genetic disease. Association studies have recently provided evidence for a link between DNA repair polymorphisms and micronucleus (MN) induction. We used the cytokinesis-block micronucleus (CBMN assay) in peripheral lymphocytes and MN test in buccal cells to investigate the effects of XRCC3 Thr241Met, ADH5 Val309Ile, and Asp353Glu polymorphisms on the frequency of genotoxicity biomarkers in individuals occupationally exposed to formaldehyde (n = 54) and unexposed workers (n = 82). XRCC3 participates in DNA double-strand break/recombination repair, while ADH5 is an important component of cellular metabolism for the elimination of formaldehyde. Exposed workers had significantly higher frequencies (P < 0.01) than controls for all genotoxicity biomarkers evaluated in this study. Moreover, there were significant associations between XRCC3 genotypes and nuclear buds, namely XRCC3 Met/Met (OR = 3.975, CI 1.053–14.998, P = 0.042) and XRCC3 Thr/Met (OR = 5.632, CI 1.673–18.961, P = 0.005) in comparison with XRCC3 Thr/Thr. ADH5 polymorphisms did not show significant effects. This study highlights the importance of integrating genotoxicity biomarkers and genetic polymorphisms in human biomonitoring studies.
Peer review: yes
URI: http://hdl.handle.net/10400.21/2581
ISSN: 0893-6692
Versão do Editor: http://onlinelibrary.wiley.com/doi/10.1002/em.21755/abstract
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