Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/2578
Título: Autoinhibition of TBCB regulates EB1-mediated microtubule dynamics
Autor: Carranza, Gerardo
Castaño, Raquel
Fanarraga, Mónica L.
Villegas, Juan Carlos
Gonçalves, João
Soares, Helena
Avila, Jesus
Marenchino, Marco
Campos-Olivas, Ramón
Montoya, Guillermo
Zabala, Juan Carlos
Palavras-chave: Cell biology
Biomedicine general
Life sciences
Biochemistry
Data: Jan-2013
Editora: Springer
Citação: Carranza G, Castaño R, Fanarraga ML, Villegas JC, Gonçalves J, Soares H, et al. Autoinhibition of TBCB regulates EB1-mediated microtubule dynamics. Cell Mol Life Sci. 2013;70(2):357-71.
Resumo: Tubulin cofactors (TBCs) participate in the folding, dimerization, and dissociation pathways of the tubulin dimer. Among them, TBCB and TBCE are two CAP-Gly domain-containing proteins that together efficiently interact with and dissociate the tubulin dimer. In the study reported here we showed that TBCB localizes at spindle and midzone microtubules during mitosis. Furthermore, the motif DEI/M-COO− present in TBCB, which is similar to the EEY/F-COO− element characteristic of EB proteins, CLIP-170, and α-tubulin, is required for TBCE–TBCB heterodimer formation and thus for tubulin dimer dissociation. This motif is responsible for TBCB autoinhibition, and our analysis suggests that TBCB is a monomer in solution. Mutants of TBCB lacking this motif are derepressed and induce microtubule depolymerization through an interaction with EB1 associated with microtubule tips. TBCB is also able to bind to the chaperonin complex CCT containing α-tubulin, suggesting that it could escort tubulin to facilitate its folding and dimerization, recycling or degradation.
Peer review: yes
URI: http://hdl.handle.net/10400.21/2578
ISSN: 1420-9071
Versão do Editor: http://rd.springer.com/article/10.1007%2Fs00018-012-1114-2
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