Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/2568
Título: In vivo and in vitro effects of RAD001 on bladder cancer
Autor: Vasconcelos-Nóbrega, Carmen
Pinto-Leite, Rosário
Arantes-Rodrigues, Regina
Ferreira, Rita
Brochado, Paulo
Cardoso, Maria L.
Palmeira, Carlos
Salvador, Alexandre
Guedes-Teixeira, Catarina I.
Colaço, Aura
Palomino, Luis F.
Lopes, Carlos
Santos, Lúcio
Oliveira, Paula A.
Palavras-chave: Bladder cancer
RAD001
Mice
N-butyl-N-(4-hydroxybutyl) nitrosamine
Bladder cancer cell lines
Proliferation
Apoptosis
mTOR
Akt expressivity
Data: Dez-2011
Editora: Elsevier
Citação: Vasconcelos-Nóbrega C, Pinto-Leite R, Arantes-Rodrigues R, Ferreira R, Brochado P, Salvador A, et al. In vivo and in vitro effects of RAD001 on bladder cancer. Urol Oncol. 2011 Dec 9. [Epub ahead of print]
Resumo: Objective: To evaluate the influence of Everolimus (RAD001) on chemically induced urothelial lesions in mice and its influence on in vitro human bladder cancer cell lines. Methods: ICR male mice were given N-butyl-N-(4-hydroxybutyl) nitrosamine in drinking water for a period of 12 weeks. Subsequently, RAD001 was administered via oral gavage, for 6 weeks. At the end of the experiment, all the animals were sacrificed and tumor development was determined by means of histopathologic evaluation; mammalian target of rapamycin (mTOR) expressivity was evaluated by immunohistochemistry. Three human bladder cancer cell lines (T24, HT1376, and 5637) were treated using a range of RAD001 concentrations. MTT assay, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), and flow cytometry were used to assess cell proliferation, apoptosis index, and cell cycle analysis, respectively. Immunoblotting analysis of 3 cell line extracts using mTOR and Akt antibodies was performed in order to study the expression of Akt and mTOR proteins and their phosphorylated forms. Results: The incidence of urothelial lesions in animals treated with RAD001 was similar to those animals not treated. RAD001 did not block T24 and HT1376 cell proliferation or induce apoptosis. A reduction in cell proliferation rate and therefore G0/G1 phase arrest, as well as a statistically significant induction of apoptosis (P 0.001), was only observed in the 5637 cell line. Conclusion: RAD001 seems not to have a significant effect on chemically induced murine bladder tumors. The effect of RAD001 on tumor proliferation and apoptosis was achieved only in superficial derived bladder cancer cell line, no effect was observed in invasive cell lines.
Peer review: yes
URI: http://hdl.handle.net/10400.21/2568
ISSN: 1873-2496
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S1078143911004066
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