Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/2453
Título: Structural characterization and immunogenicity in wild-type and immune tolerant mice of degraded recombinant human interferon Alpha2b
Autor: Hermeling, S.
Caetano, Liliana Aranha
Damen, J. M.
Slijper, M.
Schellekens, H.
Crommelin, D. J.
Jiskoot, W.
Palavras-chave: Animals
Blotting, Western
Chromatography, gel
Chromatography, high pressure liquid
Circular dichroism
Electrophoresis, polyacrylamide gel
Enzyme-linked immunosorbent assay
Glutaral/chemistry
Humans
Immune tolerance/immunology
Interferon-alpha/chemistry
Interferon-alpha/immunology
Light
Mass spectrometry
Metals
Mice
Mice, transgenic
Oxidation-reduction
Recombinant proteins
Scattering, radiation
Spectrometry, fluorescence
Spectrophotometry, ultraviolet
Surface plasmon resonance
Data: Dez-2005
Editora: Springer
Citação: Hermeling S, Caetano LA, Damen JM, Slijper M, Schellekens H, Crommelin DJ, et al. Structural characterization and immunogenicity in wild-type and immune tolerant mice of degraded recombinant human interferon Alpha2b. Pharm Res. 2005;22(12):1997-2006.
Resumo: Purpose: This study was conducted to study the influence of protein structure on the immunogenicity in wild-type and immune tolerant mice of well-characterized degradation products of recombinant human interferon alpha2b (rhIFNα2b). Methods: RhIFNα2b was degraded by metal-catalyzed oxidation (M), cross-linking with glutaraldehyde (G), oxidation with hydrogen peroxide (H), and incubation in a boiling water bath (B). The products were characterized with UV absorption, circular dichroism and fluorescence spectroscopy, gel permeation chromatography, reverse-phase high-pressure liquid chromatography, sodium dodecyl sulfate polyacrylamide gel electrophoresis, Western blotting, and mass spectrometry. The immunogenicity of the products was evaluated in wild-type mice and in transgenic mice immune tolerant for hIFNα2. Serum antibodies were detected by enzyme-linked immunosorbent assay or surface plasmon resonance. Results: M-rhIFNα2b contained covalently aggregated rhIFNα2b with three methionines partly oxidized to methionine sulfoxides. G-rhIFNα2b contained covalent aggregates and did not show changes in secondary structure. H-rhIFNα2b was only chemically changed with four partly oxidized methionines. B-rhIFNα2b was largely unfolded and heavily aggregated. Nontreated (N) rhIFNα2b was immunogenic in the wild-type mice but not in the transgenic mice, showing that the latter were immune tolerant for rhIFNα2b. The anti-rhIFNα2b antibody levels in the wild-type mice depended on the degradation product: M-rhIFNα2b > H-rhIFNα2b ∼ N-rhIFNα2b ≫ B-rhIFNα2b; G-rhIFNα2b did not induce anti-rhIFNα2b antibodies. In the transgenic mice, only M-rhIFNα2b could break the immune tolerance. Conclusions: RhIFNα2b immunogenicity is related to its structural integrity. Moreover, the immunogenicity of aggregated rhIFNα2b depends on the structure and orientation of the constituent protein molecules and/or on the aggregate size.
Peer review: yes
URI: http://hdl.handle.net/10400.21/2453
ISSN: 1573-904X
Versão do Editor: http://link.springer.com/article/10.1007%2Fs11095-005-8177-9
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