Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/2083
Título: The expression of tubulin cofactor A (TBCA) is regulated by a noncoding antisense Tbca RNA during testis maturation
Autor: Nolasco, Sofia
Bellido, Javier
Gonçalves, João
Zabala, Juan Carlos
Soares, Helena
Tavares, Alexandra
Palavras-chave: Amino acid sequence
Animals
Base sequence
Cell line
Chromosomes, mammalian/genetics
Gene expression regulation, developmental
Gene knockdown techniques
Genome/genetics
Male
Mice
Microtubule-associated proteins/chemistry
Microtubule-associated proteins/genetics
Microtubule-associated proteins/metabolism
Models, molecular
Molecular chaperones/chemistry
Molecular chaperones/genetics
Molecular chaperones/metabolism
Molecular sequence data
RNA, Antisense/genetics
RNA, Antisense/metabolism
RNA, Messenger/genetics
RNA, Messenger/metabolism
RNA, Untranslated/genetics
RNA, Untranslated/metabolism
Spermatocytes/metabolism
Spermatogenesis/genetics
Testis/growth & development
Testis/metabolism
Transcription, Genetic
Data: Ago-2012
Editora: PLoS
Citação: Nolasco S, Bellido J, Gonçalves J, Tavares A, Zabala JC, Soares H. The expression of tubulin cofactor A (TBCA) is regulated by a noncoding antisense Tbca RNA during testis maturation. PLoS ONE. 2012;7(8):e42536.
Resumo: Abstract - Recently, long noncoding RNAs have emerged as pivotal molecules for the regulation of coding genes' expression. These molecules might result from antisense transcription of functional genes originating natural antisense transcripts (NATs) or from transcriptional active pseudogenes. TBCA interacts with β-tubulin and is involved in the folding and dimerization of new tubulin heterodimers, the building blocks of microtubules. Methodology/Principal findings: We found that the mouse genome contains two structurally distinct Tbca genes located in chromosomes 13 (Tbca13) and 16 (Tbca16). Interestingly, the two Tbca genes albeit ubiquitously expressed, present differential expression during mouse testis maturation. In fact, as testis maturation progresses Tbca13 mRNA levels increase progressively, while Tbca16 mRNA levels decrease. This suggests a regulatory mechanism between the two genes and prompted us to investigate the presence of the two proteins. However, using tandem mass spectrometry we were unable to identify the TBCA16 protein in testis extracts even in those corresponding to the maturation step with the highest levels of Tbca16 transcripts. These puzzling results led us to re-analyze the expression of Tbca16. We then detected that Tbca16 transcription produces sense and natural antisense transcripts. Strikingly, the specific depletion by RNAi of these transcripts leads to an increase of Tbca13 transcript levels in a mouse spermatocyte cell line. Conclusions/Significance: Our results demonstrate that Tbca13 mRNA levels are post-transcriptionally regulated by the sense and natural antisense Tbca16 mRNA levels. We propose that this regulatory mechanism operates during spermatogenesis, a process that involves microtubule rearrangements, the assembly of specific microtubule structures and requires critical TBCA levels.
Peer review: yes
URI: http://hdl.handle.net/10400.21/2083
ISSN: 1932-6203
Versão do Editor: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0042536
Aparece nas colecções:ESTeSL - Artigos

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