Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/1722
Título: DRhoGEF2 regulates cellular tension and cell pulsations in the Amnioserosa during Drosophila dorsal closure
Autor: Azevedo, Dulce
Antunes, Marco
Prag, Soren
Ma, Xiaoyan
Hacker, Udo
Brodland, G. Wayne
Hutson, M. Shane
Solon, Jerome
Jacinto, António
Palavras-chave: DRhoGEF2
Data: 2011
Editora: PLoS
Citação: Azevedo D, Antunes M, Prag S, Ma X, Hacker U, Brodland GW, et al. DRhoGEF2 regulates cellular tension and cell pulsations in the Amnioserosa during Drosophila dorsal closure. PLoS ONE. 2011;6(9):e23964.
Resumo: Coordination of apical constriction in epithelial sheets is a fundamental process during embryogenesis. Here, we show that DRhoGEF2 is a key regulator of apical pulsation and constriction of amnioserosal cells during Drosophila dorsal closure. Amnioserosal cells mutant for DRhoGEF2 exhibit a consistent decrease in amnioserosa pulsations whereas overexpression of DRhoGEF2 in this tissue leads to an increase in the contraction time of pulsations. We probed the physical properties of the amnioserosa to show that the average tension in DRhoGEF2 mutant cells is lower than wild-type and that overexpression of DRhoGEF2 results in a tissue that is more solid-like than wild-type. We also observe that in the DRhoGEF2 overexpressing cells there is a dramatic increase of apical actomyosin coalescence that can contribute to the generation of more contractile forces, leading to amnioserosal cells with smaller apical surface than wild-type. Conversely, in DRhoGEF2 mutants, the apical actomyosin coalescence is impaired. These results identify DRhoGEF2 as an upstream regulator of the actomyosin contractile machinery that drives amnioserosa cells pulsations and apical constriction.
Peer review: yes
URI: http://hdl.handle.net/10400.21/1722
ISSN: 1932-6203
Versão do Editor: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023964
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